Reported in Nature these days, one of the largest sets of high quality human induced pluripotent come cell lines from healthy individuals has been produced by the consortium involving the Wellcome Trust Sanger Institute. Comprehensively annotated and available for independent research, the hundreds of stem cellular lines are a powerful resource for scientists studying human growth and disease.
With collaborative partners from King’s College London, the European Bioinformatics Institute, the University of Dundee and the University associated with Cambridge, the study also investigates in unprecedented detail the particular extensive variation between stem cells from different healthful people.
Technological advancements have made it achievable to take an adult cell and use specific growth situations to turn back the clock — returning it for an early embryonic state. This results in an induced pluripotent stem cell (iPSC), which can develop into any type of cell in your body. These iPSCs have huge scientific potential for studying the particular development and the impact of diseases including cancer, Alzheimer’s, and heart disease.
However , the process of creating a good iPSC is long and complicated and few laboratories have the facilities to characterise their cells in a way that makes it useful for other scientists to use.
The Human Caused Pluripotent Stem Cell Initiative (HipSci) project used standard methods to generate iPSCs on a large scale to study right after between healthy people. Reference sets of stem tissues were generated from skin biopsies donated by 301 healthy volunteers, creating multiple stem cell lines through each person.
The researchers created 711 cellular lines and generated detailed information about their genome, the particular proteins expressed in them, and the cell biology of each cellular line. Lines and data generated by this effort are available to academic researchers and industry.
Dr Daniel Gaffney, a lead author on the papers, from the Wellcome Trust Sanger Institute, said: “We have got created a comprehensive, high quality reference set of human induced pluripotent stem cell lines from healthy volunteers. Each of these originate cell lines has been extensively characterised and made available to the particular wider research community along with the annotation data. This source is a stepping stone for researchers to make better cellular models of many diseases, because they can study disease danger in many cell types, including those that are normally inaccessible. inch
By creating more than one stem cell series from each healthy individual, the researchers were able to figure out the similarity of stem cell lines from the exact same person.
Prof Fiona Watt, a guide author on the paper and co-principal investigator of HipSci, from King’s College London, said: “Many other attempts to create stem cells focus on rare diseases. In our research, stem cells have been produced from hundreds of healthy volunteers to analyze common genetic variation. We were able to show similar features of iPS cells from the same person, and exposed that up to 46 per cent of the differences we noticed in iPS cells were due to differences between people. These data will allow researchers to put disease variations within context with healthy people. ”
The particular project, which has taken 4 years to complete, required the multidisciplinary approach with many different collaborators, who specialised in various aspects of creating the cell lines and characterising the information.
Dr Oliver Stegle, a lead writer on the paper, from the European Bioinformatics Institute, said: “This study was only possible due to the large scale, organized production and characterisation of the stem cell lines. To assist us to understand the different properties of the cells, we gathered extensive data on multiple molecular layers, from the genome of the lines to their cell biology. This type of phenotyping needed a whole facility rather than just a single lab, and will give a huge resource to other scientists. Already, the data being produced have helped to gain a clearer picture of such a typical human iPSC cell looks like. ”
Dr Michael Dunn, Head of Genetics and Molecular Sciences at Wellcome, said: “This is the fantastic consequence of many years of work to create a national resource of high quality, well-characterised individual induced pluripotent stem cells. This has been a significant accomplishment made possible by the collaboration of researchers across the country with shared funding provided by Wellcome and the MRC. It will help to provide the information base to underpin a huge amount of future research into the associated with our genes on health and disease. By ensuring this particular resource is openly available to all, we hope that it may pave the way for many more fascinating discoveries. ”