Author: Stem Cells Daily

CRISPR Screens Stem Cell Genome, Charts Essentialome

Scientists at the Hebrew University of Jerusalem have generated an atlas of genes—the essentialome —that are essential for the normal growth and maintenance of human pluripotentent stem cells (hPSCs). The team, led by Nissim Benvenisty, M.D., Ph.D., director of the Azrieli Center for Stem Cells and Genetic Research and the Herbert Cohn Chair in Cancer Research, carried out a genome-wide CRISPR-Cas9 loss-of-function screen on haploid human embryonic stem cell (hESCs) to highlight which of more than 18,000 genes are necessary for growth and survival of the pluripotent cells and which genes restrict cell growth. “Our screen revealed the essentialome of hPSC-specific genes, and highlighted the main pathways that regulate the growth of these cells,” the researchers write in their published paper in Nature Cell Biology . The findings also uncovered opposing roles for tumor suppressor and oncogenes, evaluated the role of genes for hereditary disorders in early human...

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Scientists generate an atlas of the human genome using stem cells

Scientists have generated an atlas of the human genome that illuminates the roles our genes play in health and disease. The gene atlas, created using a state-of-the-art gene editing technology and human embryonic stem cells, enables a new functional view on how we study the human genome, and provides a tool that will change how we study and treat cancer and genetic...

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New cell therapy aids heart recovery — without implanting cells

Medical researchers have designed a creative new approach to help injured hearts regenerate by applying extracellular vesicles secreted by cardiomyocytes rather than implanting the cells. The study shows that the cardiomyocytes derived from human pluripotent stem cells (derived in turn from a small sample of blood) could be a powerful, untapped source of therapeutic microvesicles that could lead to safe and effective treatments of damaged...

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