Surfactant effects on the viability and function of human mesenchymal stem cells: in vitro and in vivo assessment

Our study was approved by the Animal Care and Use Committee at Taipei Medical University. Time-dated pregnant Sprague-Dawley rats were housed in individual cages with 12-h light-dark cycles. Within 12 hours of birth, litters were pooled and randomly redistributed to the newly delivered mothers, and the pups were then randomly assigned to room air (RA) or oxygen-enriched atmosphere (O2) treatment. The pups in O2 treatment subgroups were reared in an atmosphere containing 85% O2 from postnatal days 1 to 14. The pups in RA control subgroups were reared in normal RA for 14 days. To avoid oxygen toxicity in the nursing mothers, they were rotated between the O2 treatment and RA control litters every 24 hours. An oxygen-rich atmosphere was maintained in a transparent 40 × 50 × 60-cm plexiglass chamber receiving O2 continuously at 4 L/min. Oxygen levels were monitored using a ProOx P110 monitor (Bio-Spherix; Redfield, NY, USA). For intratracheal administration, the rat pups were anesthetized with 2% isoflurane (Halocarbon Laboratories, River Edge, NJ, USA), positioned against an angled restraining stand, and exposed to locate the trachea. Human MSCs (1 × 105 cells) in 30 μl of normal saline (NS); 10 μl of a surfactant (Survanta, Abbvie, North Chicago, IL, USA), corresponding to approximately 35 mg/kg of phospholipids, diluted in 20 μl of NS; and 10 μl of the surfactant and MSCs (1 × 105 cells) diluted in 20 μl of NS were administered into the rat trachea by using a 30-gauge syringe (Hamilton Company,...

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